human b ngf antibody Search Results


93
Developmental Studies Hybridoma Bank monoclonal antibodies against brain derived neurotrophic factors bdnf
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Monoclonal Antibodies Against Brain Derived Neurotrophic Factors Bdnf, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
R&D Systems ngf neutralizing antibody
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Ngf Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Santa Cruz Biotechnology anti nt 3
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Anti Nt 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti l1cam
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Anti L1cam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
R&D Systems human ngf
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Human Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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98
Abcam rabbit polyclonal
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Rabbit Polyclonal, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
R&D Systems human b ngf antibody
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Human B Ngf Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bio-Techne corporation human beta-ngf antibody
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Human Beta Ngf Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Thermo Fisher biotinylated mouse anti-human cd171 antibody (clone 5g3)
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Biotinylated Mouse Anti Human Cd171 Antibody (Clone 5g3), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Santa Cruz Biotechnology anti nt-3
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Anti Nt 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
R&D Systems p75 ntr
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
P75 Ntr, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Cedarlane rabbit polyclonal ngf antibody mc 51
HEK-CM stimulates host's cell survival factors <t>BDNF</t> and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.
Rabbit Polyclonal Ngf Antibody Mc 51, supplied by Cedarlane, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


HEK-CM stimulates host's cell survival factors BDNF and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.

Journal: Journal of Toxicology

Article Title: Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study

doi: 10.1155/2014/194967

Figure Lengend Snippet: HEK-CM stimulates host's cell survival factors BDNF and Bcl-2. (a) Photomicrograph represents the expression of BDNF in different treatment groups. Scale Bar 200 μ m. (b) Bar diagram signifies the fold changes in BDNF fluorescence intensity as compared to NC. (c) MTT Assay. Bar diagram represents the viable cells in different treatment groups. Neutralization of BDNF in HEK-CM diminishes the neuroprotective potential of HEK-CM against excitotoxicity. Nevertheless, significant neuroprotection was observed in KA + CM with anti-BDNF group as compared to KA group suggesting that other growth factors/cytokines in CM might be contributing to the observed neuroprotection. Normal control (NC), normal control exposed to HEK conditioned medium (CM), Kainic acid alone treated (KA), Kainic acid and HEK conditioned medium cotreated (KA + CM), and Kainic acid and BDNF neutralized HEK conditioned medium cotreated (KA + CM with anti-BDNF). Data represented as mean ± SEM. * indicates comparison between NC and treatment; + indicates comparison between KA lesion and treatment; * P < 0.05; *** P < 0.001; +++ P < 0.001.

Article Snippet: Following several washes in PBS, cells were incubated in blocking buffer (3% bovine serum albumin in PBS) for 30 minutes at room temperature and were incubated with primary monoclonal antibodies against brain derived neurotrophic factors (BDNF) (1 : 200; Developmental studies hybridoma bank, Iowa, USA) overnight at 4°C.

Techniques: Expressing, Fluorescence, MTT Assay, Neutralization, Control, Comparison